111In-labeled CD34+ hematopoietic progenitor cells in a rat myocardial infarction model.
Identifieur interne : 003150 ( Main/Exploration ); précédent : 003149; suivant : 003151111In-labeled CD34+ hematopoietic progenitor cells in a rat myocardial infarction model.
Auteurs : RBID : pubmed:15001696English descriptors
- KwdEn :
- Animals, Antigens, CD34 (metabolism), Disease Models, Animal, Feasibility Studies, Female, Hematopoietic Stem Cell Transplantation (methods), Hematopoietic Stem Cells (metabolism), Hematopoietic Stem Cells (pathology), Hematopoietic Stem Cells (radionuclide imaging), Humans, Isotope Labeling, Myocardial Infarction (metabolism), Myocardial Infarction (pathology), Myocardial Infarction (radionuclide imaging), Myocardial Infarction (surgery), Organometallic Compounds (diagnostic use), Oxyquinoline (analogs & derivatives), Oxyquinoline (diagnostic use), Radiopharmaceuticals (diagnostic use), Rats, Rats, Nude, Treatment Outcome.
- MESH :
- chemical , analogs & derivatives : Oxyquinoline.
- chemical , diagnostic use : Organometallic Compounds, Oxyquinoline, Radiopharmaceuticals.
- chemical , metabolism : Antigens, CD34.
- metabolism : Hematopoietic Stem Cells, Myocardial Infarction.
- methods : Hematopoietic Stem Cell Transplantation.
- pathology : Hematopoietic Stem Cells, Myocardial Infarction.
- radionuclide imaging : Hematopoietic Stem Cells, Myocardial Infarction.
- surgery : Myocardial Infarction.
- Animals, Disease Models, Animal, Feasibility Studies, Female, Humans, Isotope Labeling, Rats, Rats, Nude, Treatment Outcome.
Abstract
Transplantation of progenitor cells (PCs) has been shown to improve neovascularization and left ventricular function after myocardial ischemia. The fate of transplanted PCs has been monitored by fluorescence labeling or by genetic modifications introducing reporter genes. However, these techniques are limited by the need to kill the experimental animal. The aim of this study was to radiolabel CD34(+) hematopoietic PCs (HPCs) with (111)In-oxine and to evaluate the feasibility of this in vivo method for monitoring myocardial homing of transplanted cells in a rat myocardial infarction model.
PubMed: 15001696
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">111In-labeled CD34+ hematopoietic progenitor cells in a rat myocardial infarction model.</title><author><name sortKey="Brenner, Winfried" uniqKey="Brenner W">Winfried Brenner</name><affiliation wicri:level="3"><nlm:affiliation>Department of Nuclear Medicine, University Hospital Kiel, Kiel, Germany. winbren_2000@yahoo.com</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Nuclear Medicine, University Hospital Kiel, Kiel</wicri:regionArea><placeName><region type="land" nuts="2">Schleswig-Holstein</region><settlement type="city">Kiel</settlement></placeName></affiliation></author><author><name sortKey="Aicher, Alexandra" uniqKey="Aicher A">Alexandra Aicher</name></author><author><name sortKey="Eckey, Thomas" uniqKey="Eckey T">Thomas Eckey</name></author><author><name sortKey="Massoudi, Schirin" uniqKey="Massoudi S">Schirin Massoudi</name></author><author><name sortKey="Zuhayra, Maaz" uniqKey="Zuhayra M">Maaz Zuhayra</name></author><author><name sortKey="Koehl, Ulrike" uniqKey="Koehl U">Ulrike Koehl</name></author><author><name sortKey="Heeschen, Christopher" uniqKey="Heeschen C">Christopher Heeschen</name></author><author><name sortKey="Kampen, Willm U" uniqKey="Kampen W">Willm U Kampen</name></author><author><name sortKey="Zeiher, Andreas M" uniqKey="Zeiher A">Andreas M Zeiher</name></author><author><name sortKey="Dimmeler, Stefanie" uniqKey="Dimmeler S">Stefanie Dimmeler</name></author><author><name sortKey="Henze, Eberhard" uniqKey="Henze E">Eberhard Henze</name></author></titleStmt><publicationStmt><date when="2004">2004</date><idno type="RBID">pubmed:15001696</idno><idno type="pmid">15001696</idno><idno type="wicri:Area/Main/Corpus">003261</idno><idno type="wicri:Area/Main/Curation">003261</idno><idno type="wicri:Area/Main/Exploration">003150</idno></publicationStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antigens, CD34 (metabolism)</term><term>Disease Models, Animal</term><term>Feasibility Studies</term><term>Female</term><term>Hematopoietic Stem Cell Transplantation (methods)</term><term>Hematopoietic Stem Cells (metabolism)</term><term>Hematopoietic Stem Cells (pathology)</term><term>Hematopoietic Stem Cells (radionuclide imaging)</term><term>Humans</term><term>Isotope Labeling</term><term>Myocardial Infarction (metabolism)</term><term>Myocardial Infarction (pathology)</term><term>Myocardial Infarction (radionuclide imaging)</term><term>Myocardial Infarction (surgery)</term><term>Organometallic Compounds (diagnostic use)</term><term>Oxyquinoline (analogs & derivatives)</term><term>Oxyquinoline (diagnostic use)</term><term>Radiopharmaceuticals (diagnostic use)</term><term>Rats</term><term>Rats, Nude</term><term>Treatment Outcome</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analogs & derivatives" xml:lang="en"><term>Oxyquinoline</term></keywords><keywords scheme="MESH" type="chemical" qualifier="diagnostic use" xml:lang="en"><term>Organometallic Compounds</term><term>Oxyquinoline</term><term>Radiopharmaceuticals</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Antigens, CD34</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Hematopoietic Stem Cells</term><term>Myocardial Infarction</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Hematopoietic Stem Cell Transplantation</term></keywords><keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Hematopoietic Stem Cells</term><term>Myocardial Infarction</term></keywords><keywords scheme="MESH" qualifier="radionuclide imaging" xml:lang="en"><term>Hematopoietic Stem Cells</term><term>Myocardial Infarction</term></keywords><keywords scheme="MESH" qualifier="surgery" xml:lang="en"><term>Myocardial Infarction</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Disease Models, Animal</term><term>Feasibility Studies</term><term>Female</term><term>Humans</term><term>Isotope Labeling</term><term>Rats</term><term>Rats, Nude</term><term>Treatment Outcome</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Transplantation of progenitor cells (PCs) has been shown to improve neovascularization and left ventricular function after myocardial ischemia. The fate of transplanted PCs has been monitored by fluorescence labeling or by genetic modifications introducing reporter genes. However, these techniques are limited by the need to kill the experimental animal. The aim of this study was to radiolabel CD34(+) hematopoietic PCs (HPCs) with (111)In-oxine and to evaluate the feasibility of this in vivo method for monitoring myocardial homing of transplanted cells in a rat myocardial infarction model.</div></front></TEI><pubmed><MedlineCitation Owner="NLM" Status="MEDLINE"><PMID Version="1">15001696</PMID><DateCreated><Year>2004</Year><Month>03</Month><Day>05</Day></DateCreated><DateCompleted><Year>2004</Year><Month>05</Month><Day>13</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0161-5505</ISSN><JournalIssue CitedMedium="Print"><Volume>45</Volume><Issue>3</Issue><PubDate><Year>2004</Year><Month>Mar</Month></PubDate></JournalIssue><Title>Journal of nuclear medicine : official publication, Society of Nuclear Medicine</Title><ISOAbbreviation>J. Nucl. Med.</ISOAbbreviation></Journal><ArticleTitle>111In-labeled CD34+ hematopoietic progenitor cells in a rat myocardial infarction model.</ArticleTitle><Pagination><MedlinePgn>512-8</MedlinePgn></Pagination><Abstract><AbstractText Label="UNLABELLED">Transplantation of progenitor cells (PCs) has been shown to improve neovascularization and left ventricular function after myocardial ischemia. The fate of transplanted PCs has been monitored by fluorescence labeling or by genetic modifications introducing reporter genes. However, these techniques are limited by the need to kill the experimental animal. The aim of this study was to radiolabel CD34(+) hematopoietic PCs (HPCs) with (111)In-oxine and to evaluate the feasibility of this in vivo method for monitoring myocardial homing of transplanted cells in a rat myocardial infarction model.</AbstractText><AbstractText Label="METHODS" NlmCategory="METHODS">Human HPCs were isolated from mobilized peripheral blood and labeled with (111)In-oxine. Labeled HPCs were injected into the cavity of the left ventricle in nude rats 24 h after induction of myocardial infarction (n = 4) or sham operation (n = 4). Scintigraphic images were acquired up to 96 h after HPC injection. After animals were killed, tissue samples of various organs were harvested to calculate tissue-specific activity and for immunostaining.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">Labeling efficiency of HPCs was 32% +/- 11%. According to trypan-blue staining, viability of radiolabeled HPCs was impaired by 30% after 48 and 96 h in comparison with unlabeled cells, whereas proliferation and differentiation of HPCs was nullified after 7 d, as assessed by colony-forming assays. After injection of HPCs, the specific activity ratio of heart to peripheral muscle tissue increased from 1.10 +/- 0.32 in sham-operated rats to 2.47 +/- 0.92 (P = 0.020) in infarcted rats. However, the overall radioactivity detected in the heart was only about 1%. A transient high lung uptake of 17% +/- 6% was observed within the first hour after infusion of HPCs. At 24 h after injection, the initial lung activity had shifted toward liver, kidneys, and spleen, resulting in an increase of radioactivity in these organs from 37% +/- 6% to 57% +/- 5%.</AbstractText><AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">Radiolabeling with (111)In-oxine is a feasible in vivo method for monitoring transplanted HPCs in a rat myocardial infarction model. The potential to detect differences in myocardial homing between infarcted and normal hearts suggests that this method may provide a noninvasive imaging approach for clinical trials using transplanted HPCs in patients. Our findings, however, also demonstrated a negative effect of (111)In-oxine on cellular function, which resulted in complete impairment of HPC proliferation and differentiation. For future trials in stem cell imaging with (111)In-oxine, therefore, it will be mandatory to carefully check for radiation-induced cell damage.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Brenner</LastName><ForeName>Winfried</ForeName><Initials>W</Initials><Affiliation>Department of Nuclear Medicine, University Hospital Kiel, Kiel, Germany. winbren_2000@yahoo.com</Affiliation></Author><Author ValidYN="Y"><LastName>Aicher</LastName><ForeName>Alexandra</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Eckey</LastName><ForeName>Thomas</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>Massoudi</LastName><ForeName>Schirin</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Zuhayra</LastName><ForeName>Maaz</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Koehl</LastName><ForeName>Ulrike</ForeName><Initials>U</Initials></Author><Author ValidYN="Y"><LastName>Heeschen</LastName><ForeName>Christopher</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Kampen</LastName><ForeName>Willm U</ForeName><Initials>WU</Initials></Author><Author ValidYN="Y"><LastName>Zeiher</LastName><ForeName>Andreas M</ForeName><Initials>AM</Initials></Author><Author ValidYN="Y"><LastName>Dimmeler</LastName><ForeName>Stefanie</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Henze</LastName><ForeName>Eberhard</ForeName><Initials>E</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType>Evaluation Studies</PublicationType><PublicationType>Journal Article</PublicationType><PublicationType>Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>J Nucl Med</MedlineTA><NlmUniqueID>0217410</NlmUniqueID><ISSNLinking>0161-5505</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Antigens, CD34</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Organometallic Compounds</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Radiopharmaceuticals</NameOfSubstance></Chemical><Chemical><RegistryNumber>14514-42-2</RegistryNumber><NameOfSubstance>indium oxine</NameOfSubstance></Chemical><Chemical><RegistryNumber>5UTX5635HP</RegistryNumber><NameOfSubstance>Oxyquinoline</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Antigens, CD34</DescriptorName><QualifierName MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Disease Models, Animal</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Feasibility Studies</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Female</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Hematopoietic Stem Cell Transplantation</DescriptorName><QualifierName MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Hematopoietic Stem Cells</DescriptorName><QualifierName MajorTopicYN="Y">metabolism</QualifierName><QualifierName MajorTopicYN="N">pathology</QualifierName><QualifierName MajorTopicYN="Y">radionuclide imaging</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Isotope Labeling</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Myocardial Infarction</DescriptorName><QualifierName MajorTopicYN="N">metabolism</QualifierName><QualifierName MajorTopicYN="N">pathology</QualifierName><QualifierName MajorTopicYN="Y">radionuclide imaging</QualifierName><QualifierName MajorTopicYN="Y">surgery</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Organometallic Compounds</DescriptorName><QualifierName MajorTopicYN="Y">diagnostic use</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Oxyquinoline</DescriptorName><QualifierName MajorTopicYN="Y">analogs & derivatives</QualifierName><QualifierName MajorTopicYN="Y">diagnostic use</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Radiopharmaceuticals</DescriptorName><QualifierName MajorTopicYN="Y">diagnostic use</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Rats</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Rats, Nude</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Treatment Outcome</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2004</Year><Month>3</Month><Day>6</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2004</Year><Month>5</Month><Day>14</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2004</Year><Month>3</Month><Day>6</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">15001696</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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